diff --git a/core/core.services.yml b/core/core.services.yml
index dd8d66a374..fbb93c57c6 100644
--- a/core/core.services.yml
+++ b/core/core.services.yml
@@ -1163,13 +1163,6 @@ services:
     class: Drupal\Core\Entity\EntityAccessCheck
     tags:
       - { name: access_check, applies_to: _entity_access }
-  access_check.entity_bundles:
-    # Deprecated in drupal:9.2.0 and is removed from drupal:10.0.0. Specify the
-    # list of bundles in the entity parameter, under "bundle" key as a sequence,
-    # instead. See https://www.drupal.org/node/3155569.
-    class: Drupal\Core\Entity\EntityBundleAccessCheck
-    tags:
-      - { name: access_check, applies_to: _entity_bundles }
   access_check.entity_create:
     class: Drupal\Core\Entity\EntityCreateAccessCheck
     arguments: ['@entity_type.manager']
diff --git a/core/lib/Drupal/Core/Entity/EntityType.php b/core/lib/Drupal/Core/Entity/EntityType.php
index 527930c067..629865d126 100644
--- a/core/lib/Drupal/Core/Entity/EntityType.php
+++ b/core/lib/Drupal/Core/Entity/EntityType.php
@@ -431,14 +431,6 @@ public function entityClassImplements($interface) {
     return is_subclass_of($this->getClass(), $interface);
   }
 
-  /**
-   * {@inheritdoc}
-   */
-  public function isSubclassOf($class) {
-    @trigger_error(__METHOD__ . '() is deprecated in drupal:8.3.0 and is removed from drupal:10.0.0. Use Drupal\Core\Entity\EntityTypeInterface::entityClassImplements() instead. See https://www.drupal.org/node/2842808', E_USER_DEPRECATED);
-    return $this->entityClassImplements($class);
-  }
-
   /**
    * {@inheritdoc}
    */
diff --git a/core/lib/Drupal/Core/Entity/EntityTypeInterface.php b/core/lib/Drupal/Core/Entity/EntityTypeInterface.php
index 5e31b9675e..b63e677e6e 100644
--- a/core/lib/Drupal/Core/Entity/EntityTypeInterface.php
+++ b/core/lib/Drupal/Core/Entity/EntityTypeInterface.php
@@ -360,23 +360,6 @@ public function setAccessClass($class);
    */
   public function entityClassImplements($interface);
 
-  /**
-   * Indicates if the entity type is a subclass of the given class or interface.
-   *
-   * @param string $class
-   *   The class or interface to check.
-   *
-   * @return bool
-   *   TRUE if the entity type is a subclass of the class or interface.
-   *
-   * @deprecated in drupal:8.3.0 and is removed from drupal:10.0.0.
-   *   Use Drupal\Core\Entity\EntityTypeInterface::entityClassImplements()
-   *   instead.
-   *
-   * @see https://www.drupal.org/node/2842808
-   */
-  public function isSubclassOf($class);
-
   /**
    * Sets the handlers for a given type.
    *
diff --git a/core/lib/Drupal/Core/Entity/Query/Sql/Query.php b/core/lib/Drupal/Core/Entity/Query/Sql/Query.php
index ad66a42972..1ba8c1f51c 100644
--- a/core/lib/Drupal/Core/Entity/Query/Sql/Query.php
+++ b/core/lib/Drupal/Core/Entity/Query/Sql/Query.php
@@ -137,8 +137,7 @@ protected function prepare() {
     }
 
     if (is_null($this->accessCheck)) {
-      $this->accessCheck = TRUE;
-      @trigger_error('Relying on entity queries to check access by default is deprecated in drupal:9.2.0 and an error will be thrown from drupal:10.0.0. Call \Drupal\Core\Entity\Query\QueryInterface::accessCheck() with TRUE or FALSE to specify whether access should be checked. See https://www.drupal.org/node/3201242', E_USER_DEPRECATED);
+      throw new QueryException('Entity query must specify whether access should be checked.');
     }
     if ($this->accessCheck) {
       $this->sqlQuery->addTag($this->entityTypeId . '_access');
diff --git a/core/lib/Drupal/Core/Entity/RevisionLogEntityTrait.php b/core/lib/Drupal/Core/Entity/RevisionLogEntityTrait.php
index 4585155c42..4c4088eda9 100644
--- a/core/lib/Drupal/Core/Entity/RevisionLogEntityTrait.php
+++ b/core/lib/Drupal/Core/Entity/RevisionLogEntityTrait.php
@@ -123,24 +123,6 @@ public function setRevisionLogMessage($revision_log_message) {
     return $this;
   }
 
-  /**
-   * Gets the name of a revision metadata field.
-   *
-   * @param \Drupal\Core\Entity\EntityTypeInterface $entity_type
-   *   A content entity type definition.
-   * @param string $key
-   *   The revision metadata key to get, must be one of 'revision_created',
-   *   'revision_user' or 'revision_log_message'.
-   *
-   * @return string
-   *   The name of the field for the specified $key.
-   */
-  protected static function getRevisionMetadataKey(EntityTypeInterface $entity_type, $key) {
-    @trigger_error(static::class . 'getRevisionMetadataKey() is deprecated in drupal:9.0.0 and is removed from drupal:10.0.0. Use $entity_type->getRevisionMetadataKey() instead. See: https://www.drupal.org/node/2831499', E_USER_DEPRECATED);
-    /** @var \Drupal\Core\Entity\ContentEntityTypeInterface $entity_type */
-    return $entity_type->getRevisionMetadataKey($key);
-  }
-
   /**
    * Gets the entity type definition.
    *
diff --git a/core/tests/Drupal/KernelTests/Core/Entity/EntityQueryTest.php b/core/tests/Drupal/KernelTests/Core/Entity/EntityQueryTest.php
index bf35a517f3..e119f6b97c 100644
--- a/core/tests/Drupal/KernelTests/Core/Entity/EntityQueryTest.php
+++ b/core/tests/Drupal/KernelTests/Core/Entity/EntityQueryTest.php
@@ -3,6 +3,7 @@
 namespace Drupal\KernelTests\Core\Entity;
 
 use Drupal\Core\Database\Database;
+use Drupal\Core\Entity\Query\QueryException;
 use Drupal\entity_test\Entity\EntityTest;
 use Drupal\entity_test\Entity\EntityTestMulRev;
 use Drupal\field\Entity\FieldConfig;
@@ -1358,11 +1359,10 @@ public function testToString() {
 
   /**
    * Test the accessCheck method is called.
-   *
-   * @group legacy
    */
   public function testAccessCheckSpecified() {
-    $this->expectDeprecation('Relying on entity queries to check access by default is deprecated in drupal:9.2.0 and an error will be thrown from drupal:10.0.0. Call \Drupal\Core\Entity\Query\QueryInterface::accessCheck() with TRUE or FALSE to specify whether access should be checked. See https://www.drupal.org/node/3201242');
+    $this->expectException(QueryException::class);
+    $this->expectExceptionMessage('Entity query must specify whether access should be checked.');
     $this->storage->getQuery()->execute();
   }
 
diff --git a/core/tests/Drupal/Tests/Core/Entity/EntityBundleAccessCheckTest.php b/core/tests/Drupal/Tests/Core/Entity/EntityBundleAccessCheckTest.php
deleted file mode 100644
index c50d3049b4..0000000000
--- a/core/tests/Drupal/Tests/Core/Entity/EntityBundleAccessCheckTest.php
+++ /dev/null
@@ -1,100 +0,0 @@
-<?php
-
-namespace Drupal\Tests\Core\Entity;
-
-use Drupal\Core\Cache\Context\CacheContextsManager;
-use Drupal\Core\DependencyInjection\Container;
-use Drupal\Core\Routing\RouteMatchInterface;
-use Drupal\Core\Session\AccountInterface;
-use Drupal\node\NodeInterface;
-use Symfony\Component\HttpFoundation\InputBag;
-use Symfony\Component\HttpFoundation\ParameterBag;
-use Symfony\Component\Routing\Route;
-use Drupal\Core\Access\AccessResult;
-use Drupal\Core\Entity\EntityBundleAccessCheck;
-use Drupal\Tests\UnitTestCase;
-
-/**
- * Unit test of entity access checking system.
- *
- * @coversDefaultClass \Drupal\Core\Entity\EntityBundleAccessCheck
- *
- * @group Access
- * @group Entity
- * @group legacy
- */
-class EntityBundleAccessCheckTest extends UnitTestCase {
-
-  /**
-   * {@inheritdoc}
-   */
-  protected function setUp(): void {
-    $cache_contexts_manager = $this->prophesize(CacheContextsManager::class)->reveal();
-    $container = new Container();
-    $container->set('cache_contexts_manager', $cache_contexts_manager);
-    \Drupal::setContainer($container);
-  }
-
-  /**
-   * Data provider.
-   */
-  public function getBundleAndAccessResult() {
-    return [
-      [
-        'article',
-        'node:article',
-        AccessResult::allowed(),
-      ],
-      [
-        'page',
-        'node:article',
-        AccessResult::neutral('The entity bundle does not match the route _entity_bundles requirement.'),
-      ],
-      [
-        'page',
-        'node:article|page',
-        AccessResult::allowed(),
-      ],
-      [
-        'article',
-        'node:article|page',
-        AccessResult::allowed(),
-      ],
-      [
-        'book_page',
-        'node:article|page',
-        AccessResult::neutral('The entity bundle does not match the route _entity_bundles requirement.'),
-      ],
-    ];
-  }
-
-  /**
-   * @covers ::access
-   *
-   * @dataProvider getBundleAndAccessResult
-   */
-  public function testRouteAccess($bundle, $access_requirement, $access_result) {
-    $route = new Route('/foo/{node}', [], ['_entity_bundles' => $access_requirement], ['parameters' => ['node' => ['type' => 'entity:node']]]);
-    /** @var \Drupal\Core\Session\AccountInterface $account */
-    $account = $this->prophesize(AccountInterface::class)->reveal();
-
-    /** @var \Drupal\node\NodeInterface|\Prophecy\Prophecy\ObjectProphecy $node */
-    $node = $this->prophesize(NodeInterface::class);
-    $node->bundle()->willReturn($bundle);
-    $node->getCacheContexts()->willReturn([]);
-    $node->getCacheTags()->willReturn([]);
-    $node->getCacheMaxAge()->willReturn(-1);
-    $node = $node->reveal();
-
-    /** @var \Drupal\Core\Routing\RouteMatchInterface|\Prophecy\Prophecy\ObjectProphecy $route_match */
-    $route_match = $this->prophesize(RouteMatchInterface::class);
-    $route_match->getRawParameters()->willReturn(new InputBag(['node' => 1]));
-    $route_match->getParameters()->willReturn(new ParameterBag(['node' => $node]));
-    $route_match = $route_match->reveal();
-
-    $access_check = new EntityBundleAccessCheck();
-    $this->expectDeprecation('The Drupal\Core\Entity\EntityBundleAccessCheck is deprecated in drupal:9.2.0 and is removed from drupal:10.0.0. Specify the list of bundles in the entity parameter, under "bundle" key, as a sequence, instead. See https://www.drupal.org/node/3155569');
-    $this->assertEquals($access_result, $access_check->access($route, $route_match, $account));
-  }
-
-}